Web13 rows · Similarly, transgenic expression of the human heparin-binding EGFR, which is the diphtheria toxin receptor (DTR), in mouse cells, renders those cells susceptible, like … Diphtheria toxin is an exotoxin secreted by mainly by Corynebacterium diphtheriae but also by Corynebacterium ulcerans and Corynebacterium pseudotuberculosis. the pathogenic bacterium that causes diphtheria. The toxin gene is encoded by a prophage called corynephage β. The toxin … See more Diphtheria toxin is a single polypeptide chain of 535 amino acids consisting of two subunits linked by disulfide bridges, known as an A-B toxin. Binding to the cell surface of the B subunit (the less stable of the two subunits) allows the … See more Diphtheria toxin is extraordinarily potent. The lethal dose for humans is about 0.1 μg of toxin per kg of body weight. Death occurs through See more The drug denileukin diftitox uses diphtheria toxin as an antineoplastic agent. Resimmune is an immunotoxin that is in clinical trials in cutaneous T cell lymphoma patients. It uses diphtheria toxin (truncated by the cell binding domain) coupled to an antibody to See more 1. ^ A prophage is a virus that has inserted itself into the genome of the host bacterium. See more 1. Processing 2. The toxin binds to heparin-binding epidermal growth factor precursor (HB-EGF). 3. The complex undergoes endocytosis by the host cell. See more Diphtheria toxin was discovered in 1888 by Émile Roux and Alexandre Yersin. In 1890, Emil Adolf von Behring developed an anti-toxin based on … See more Similar to other A-B toxins, diphtheria toxin is adept at transporting exogenous proteins across mammalian cell membranes, which … See more

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WebMar 5, 2024 · The Rosa or iDTR (for inducible Diphtheria Toxin Receptor) mouse utilizes a transgene very similar to the Cre-driver lines. In this mouse (shown in Figure 3.7. 1 below) all cells throughout the mouse’s body carries the DNA sequence for the Diphtheria Toxin Receptor DTR). WebMar 3, 2024 · CD11c-dtr mice (female, 6–8 wks, Jackson) were injected with 100 ng diphtheria toxin (DT) intraperitoneally on day 3 post tumor implantation to deplete CD11c+ DCs. Wild-type C57BL/6 and DT-treated CD11c-dtr mice were injected with 200μg anti-PD-1 and 200μg anti-CTLA-4 mAb intraperitoneally on days 4 and 8 post tumor implantation. jessica webb tcu

CD206+ M2-Like Macrophages Are Essential for Successful Implantation

WebWe generated a mouse line crossing ChAT-cre and diphtheria toxin receptor (DTR)-expressing lines and injected a dose of DT (3.2 or 10 ng) to exclusively ablate SACs in the retina (Hillier et al., 2024; Care et al., 2024; Kim et al., 2024). We first examined the effect of DT injection on the retinal neurons by injecting DT in one eye, and ... WebNational Center for Biotechnology Information WebMy Research and Language Selection Sign into My Research Create My Research Account English; Help and support. Support Center Find answers to questions about products, access, use, setup, and administration.; Contact Us Have a question, idea, or some feedback? We want to hear from you. inspector linley murder