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Peasy®-blunt zero cloning kit

WebFeb 15, 2024 · 分别以杜梨cDNA和gDNA为模板扩增PbGID1家族基因。扩增程序为：95 ℃预变性2 min；95 ℃ 30 s，55 ℃ 30 s，72 ℃ 1 min，30个循环；72 ℃ 延伸 2 min。PCR产物进行胶回收后，连接至pEASY-Blunt Zero Cloning Kit克隆载体，转化大肠杆菌感受态DH5α中，挑取单克隆测序。 WebOct 8, 2024 · The amplified strips were connected with pEASY-Blunt Zero Cloning Kit after glue recovery, and the ligation products were sent to Sangon Bioengineering (Shanghai) Co., Ltd for sequencing. Whether...

拟南芥长链非编码RNA-DANA1及其在植物干旱耐受性的应用

Web7 rows · •High cloning efficiency. Positive clones up to 100%. •No blue/white selection needed. •Suitable ... WebDetails. 6027. Mighty Cloning Reagent Set (Blunt End) 20 Rxns. USD $377.00. Takara's Reagent Set for Mighty Cloning Kit (Blunt End) is designed to simply and quickly prepare PCR products for cloning into blunt-ended vectors. Because the blunting and 5'-phosphorylation reactions are done simultaneously, both blunt- and dA-ended products … bomb cyclone british columbia https://jdmichaelsrecruiting.com

Analyses of a chromosome-scale genome assembly reveal the …

WebPeasy Blunt Cloning Vector, supplied by Beijing TransGen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. stars, based on 1. Price from $ to $1999.99. peasy blunt cloning vector - by Bioz Stars , 2024-02. WebZero Blunt ® TOPO ® PCR Cloning Kits are shipped on dry ice. Kits supplied with competent cells containa box with Zero Blunt ® TOPO ® PCR Cloning reagents (Box 1) and a box with One Shot ® E. coli (Box 2). Zero Blunt ® TOPO ® PCR Cloning Kit supplied with the PureLink ® Quick Plasmid Miniprep Kit (Cat. no.K2800-02) is shipped with an ... WebBranching is speculated to contribute to the plant architecture and crop yield. As a quantitative trait, branching is regulated by multiple genes in non-heading Chinese cabbage (NHCC). Several related candidate genes have been discovered in previous studies on the branching of NHCC, but their specific functions and regulatory mechanisms still need to … bomb cyclone california today

A strategy for in-house production of a positive selection cloning ...

Category:pEASY-Blunt Zero Cloning Kit - CB501 Civic Bioscience

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Peasy®-blunt zero cloning kit

pEASY-T1 (linearized) Sequence and Map - SnapGene

WebpEASY® -T5 Zero Cloning Kit pEASY® -Blunt Zero Cloning Kit pEASY® -Uni Seamless Cloning and Assembly Kit pEASY ®-Basic Seamless Cloning and Assembly Kit Cloning Competent Cells Trans 10 Chemically Competent Cell Trans 5α Chemically Competent Cell Trans 109 Chemically Competent Cell Trans 110 Chemically Competent Cell WebpEASY-Blunt Zero Cloning Kit. pEASY ® Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain nonrecombinant vector are killed upon plating. Therefore, blue/white selection is not required. Cat. No. CB501. Specification:

Peasy®-blunt zero cloning kit

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WebStrataClone Blunt PCR Cloning Kit 5 Map for the StrataClone Blunt PCR Cloning Vector pSC-B-amp/kan Feature Nucleotide Position β-galactosidase α-fragment coding sequence (lacZ´) 1–354 Multiple cloning site (MCS) 57–197 PCR product insertion site 123 kanamycin resistance ORF 465-1256 ampicillin resistance (bla) ORF 1268–2125 WebZero Blunt™ TOPO™ PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized …

Web本发明公开了一种拟南芥长链非编码RNA‑DANA1及其在植物干旱耐受性的应用，涉及植物的基因工程领域。其中，所述的DANA1核苷酸序列如SEQ ID NO.1所示。本发明通过克隆拟南芥长链非编码RNA‑DANA1的全长转录本，并构建过表达载体获得DANA1过表达转基因植株，过表达DANA1后植物表现出失水速率减慢，对 ... WebPeasyblunt Zero Cloning Kit, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article …

Web一、 采购人名称： 石河子大学 二、 供应商名称： 石河子市北泉镇诺泰实验用品经销部 三、 采购项目名称： 石河子大学网上超市项目 四、 采购项目编号： ***** 五、 合同编号： 11n ***** 六、 合同内容： WebpEASY-Blunt Zero (linearized) Cloning vector for blunt-ended PCR products, with a lacZα-ccdB suicide gene that allows for selection of recombinant plasmids. Sequence Author: …

WebApr 11, 2024 · The PCR program consisted of 3 min at 95°C, followed by 35 cycles of 15 s at 95°C, 15 s at 55°C, and 120 s at 72°C, followed by a 5 min extension at 72°C. The PCR product was inserted into the pEASY-Blunt Simple Cloning vector (TransGen Biotech, Beijing, China) and then transformed into DH5α for sequencing.

WebSep 1, 2024 · Removal of the MCS fragment from the pJET1.2M plasmid produces the pJET1.2/blunt cloning vector ready for gene cloning. The self-made pJET1.2/blunt cloning vector exhibited a cloning... gmod the backrooms modgmod the last patientWebpEASY-T1 (linearized) TA cloning vector suitable for blue-white selection, with kanamycin and ampicillin resistance markers and a T7 promoter. Sequence Author: TransBionovo (TransGen Biotech) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7kPlasmids:Basic Cloning Vectors More Plasmid Sets … bomb cyclone cliff massWebpEASY® -Blunt Zero Cloning Kit 目录号： CB501-01 单 价： ￥780 规格： 20 rxns 60 rxns 数量： - + 说明书 载体信息 质检报告 立即下单 加入购物车 产品详情介绍 该载体通过自杀 … gmod the last of us 2Web5 min TA/Blunt-Zero Cloning Kit C601. USD$ 201.00. ClonExpress II One Step Cloning Kit C112. USD$ 217.00. Ultra-Universal TOPO Cloning Kit C603. USD$ 196.00. Mut Express MultiS Fast Mutagenesis Kit V2 C215 ... bomb cyclone current statusWebHigh cloning efficiency. Positive clones up to 100%. No blue/white selection needed. Suitable for short and large fragment cloning. Kanamycin and Ampicillin resistance genes … bomb cyclone california updateWebOct 1, 2016 · Features of the pEASY-Blunt Zero Cloning Kit. 5 minutes fast insertion of blunt-end PCR products.by topoisomerase. High cloning … bomb cyclone colorado springs