Total RNA was isolated from immature ears (2–3 cm in length) and other tissues using TRI reagent (Molecular Research Center). Northern blots using ∼12 µg total RNA of sample per lane were performed using standard procedures (see Supplementary Information). Complementary DNA for real-time … Visa mer A W22 × W22:tga1 F2 fine-mapping population of 3,106 plants that segregated for Tga1-maize versus tga1-teosinte1 was generated by self-pollinating F1 hybrids of the parental stocks. … Visa mer After genetic mapping demonstrated that tga1 lies within the BAC c126f15, we shotgun-sequenced it to 7 × coverage (see Supplementary Information). This work produced a final … Visa mer We sequenced the tga1 promoter and coding regions for a set of 16 diverse land races of maize and 12 teosinte individuals (Z. mays ssp. parviglumis) and the outgroup Zea diploperennis (GenBank AY883436–AY883558) … Visa mer The 3′ end of tga1 excluding the SBP-box was amplified by PCR from a tga1 RT–cDNA clone (Supplementary Methods). The PCR fragment was cloned into pET151/D-TOPO vector (Invitrogen) and transformed into … Visa mer WebbTable 1 Glume architecture phenotypes of isogenic lines with different genotypes at tga1 and not1 - "Evidence That the Origin of Naked Kernels During Maize Domestication Was Caused by a Single Amino Acid Substitution in tga1" Skip to search form Skip to main content Skip to account menu

The origin of the naked grains of maize Nature

Webb4 maj 2015 · In this article, we have shown that an amino acid substitution in tga1 is the causal variant that underlies the origin of the naked grains of maize as compared to the covered grains of teosinte. Although the predominant mechanism for morphological evolution may be alterations in gene expression ( Carroll 2008 ), changes in protein … Webb8 jan. 2015 · The origin of maize (Zea mays mays) in the US Southwest remains contentious, with conflicting archaeological data supporting either coastal 1–4 or … cotonificio albini albino

Corn History, Cultivation, Uses, & Description

Webb4 maj 2015 · Genetics. teosinte glume architecture1 (tga1), a member of the SBP-box gene family of transcriptional regulators, has been identified as the gene conferring naked … Webb16 jan. 2024 · Avena sativa L. subsp. nudisativa has the ability to produce naked grains. Genetic studies on the naked trait of oat began over a century ago, but the genetic and molecular factors associated with the expression of this trait have not been fully clarified. The objectives of this study were to evaluate the naked trait in two oat populations of … WebbThe most critical step in maize (Zea mays ssp. mays) domestication was the liberation of the kernel from the hardened, protective casing that envelops the kernel in the maize progenitor, teosinte. This evolutionary step exposed the kernel on the surface of the ear, such that it could readily be used by humans as a food source. magali noël stéphanie vial-noël